Input:
One or more protein sequences in fasta format. Optional OX=n can be included in the FASTA header to explicitly identify the sequence Taxa ID as n, otherwise, the Taxa ID of the sequence with the highest alignment (Bits score) will be used as the query Taxa ID. Taxa IDs can be obtained from NCBI Taxonomy name/id
Output:
- The potential deleteriousness effect of every possible amino acid mutation in the form of a heat-map matrix.
- A downloadable text file with the following columns:
- AC: Sequence id from the fasta header.
- Pos: Amino acid position.
- Ref: The reference amino acid.
- Conservation: The average LIST-S2 deleteriousness score of all possible mutations at that position.
- 20 columns one for each amino acid: The potential deleteriousness LIST-S2 score for mutating the reference amino acid to “this” amino acid. Scores are in the range [0 .. 1], where lower scores indicate more benign and higher indicate deleteriousness effect.